The main strategy of ACCeSS for addressing CO2 concentration involves coupling CO2 capture to metabolic pathways and utilizing synthetic biology to produce high-value compounds, thereby resulting in a net reduction of CO2. In Work Package 4, our goal is to contribute to ACCeSS's overarching objective by optimizing the uptake and export systems of Cyanidiales using novel technologies and methodologies.

Our central research question focuses on how the membrane transport system of Cyanidiales can be optimized to enhance nutrient import and subsequently facilitate the export of synthetic building blocks. To achieve this, we will investigate the optimization of bicarbonate importers and the exopolysaccharide secretion machinery. Additionally, we will explore the modification of heavy metal transporter systems to create microalgae capable of removing heavy metals from wastewater.

By enhancing the efficiency of bicarbonate uptake, we aim to improve the carbon capture capability of Cyanidiales, which will support the synthesis of valuable compounds through metabolic engineering. Optimizing the exopolysaccharide secretion machinery will aid in the efficient export of these compounds, making the overall process more viable and productive.

Furthermore, the project will delve into the heavy metal transporter systems of Cyanidiales. We aim to develop microalgae strains that can be deployed for bioremediation, effectively removing heavy metals from contaminated water sources. This dual approach not only addresses CO2 reduction but also contributes to environmental cleanup, showcasing the multifaceted potential of synthetic biology in solving complex ecological challenges.